Collagen Antibody Induced Arthritis Model

The clinical phenotype and pathological phenotype of CAIA are consistent with collagen induced arthritis (CIA), and mainly composed of inflammatory cell infiltration of neutrophils, macrophages and multinuclear cells. Unlike CIA, the onset of CAIA does not rely on T and B cell responses. CAIA is also a rapid model, with arthritis symptoms appearing 24-48 hours after immunization, reaching a peak in 5-7 days, with disease severity decreasing in the following days, lasting for a total of about two weeks.

Fig.1 Collagen Antibody Induced Arthritis Model

The collagen antibody induced arthritis model is shown in Fig.1A. After immunization, the arthritis score of mice in the model group increased significantly; treatment with dexamethasone decreased the arthritis score of the CAIA. (Fig.1B). The thickness changes of the left and right hind limbs of mice can be seen in Fig.1C. The redness and swelling of the mouse paws can be seen in Fig.1D. The results of HE histology staining, TRAP pathological staining and Safranine O-Fast Green of the mice showed that the bone tissue of the mice in the model group showed severe bone erosion and collagen degradation, which were alleviated after dexamethasone treatment (Fig.1E).