CCl4-induced Liver Fibrosis Models

Strain: C57BL/6 mouse and other strains

Experimental Period: 8-10 weeks

Number of lab animals: 10 per group, 6-8 groups


Data Validation

1. Sirius Red staining of liver slices in CCl4-injected mice

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Figure 1. 6-week-old C57BL/6J mice were subcutaneously injected 5 mL/kg of 20% CCl4 (twice weekly) for 10 weeks, whereas control mice were given the same volume of olive oil. Liver slices stained with Sirius Red revealed significant fibrosis in the CCl4-injected group.


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