Tissue Sample Analysis

Molecular Platform
PK/PD Platform
Pathological Diagnosis Platform
Cell-based Service Platform
Cell Line Engineering In vitro Pharmacological EvaluationTumor-bearing Mouse Models
Flow Cytometry Platform
Services and AdvantagesImmune Cell ProfilingTumor Infiltrating Lymphocyte (TILs) DetectionRed Blood Cell and Platelet DetectionTissue Sample AnalysisAntibody Binding AssayCytometric Bead Array
Imaging and Analysis Platform
Behavioral Analysis Platform

  • Thymus: Research on T-cell differentiation and development.

  • Lymph nodes: Analysis of T-cells, B-cells, and DC cells.

  • Bone marrow: Research on B cell development, red blood cell development, and hematopoietic stem cells.

  • Kidney: Analysis of inflammatory cell infiltration.

  • Lung: Analysis of immune cell infiltration in mouse lung tissue in tumors or inflammatory conditions.

  • Skin: Analysis of cellular inflammatory infiltration.

  • Brain: Analysis of the infiltration of inflammatory cells within the organization.


Experimental Case Study 1: Thymus T cell development.


Population

Gating Step

Th Cells

Live single

mCD4+

mCD8-

 /

 /

Tc Cells

Live single

mCD4-

mCD8+

 /

 /

DP Cells

Live single

mCD4+

mCD8+

 /

/

DN Cells

Live single

mCD4-

mCD8-

 /

/

DN2 Cells

Live single

mCD4-

mCD8-

mCD44+

mCD25+

DN3 Cells

Live single

mCD4-

mCD8-

mCD44-

mCD25+

DN1 Cells

Live single

mCD4-

mCD8-

mCD44+

mCD25-

DN4 Cells

Live single

mCD4-

mCD8-

mCD44-

mCD25-

图片5.png


Experimental Case Study 2: Analysis of Renal immune cell (12-color panel)


Population

Gating Step

Total Leukocytes

Live single

mCD45+

/

/

/

Neutrophils

Live single

mCD45+

mCD11b+

Ly6G+

/

Monocytes

Live single

Not Neutrophils

mCD11b+

Ly6C hi

/

Eosinophils

Live single

Not monocytes

mCD11b+

SSC-H hi

/

Macrophage

Live single

Not eosinophils

mCD11b+

F4/80+

/

DC Cells

Live single

Not eosinophils

mCD11b+

mCD11c+

/

T Cells

Live single

Not eosinophils

mCD3+

mCD335-

/

B Cells

Live single

Not eosinophils

mCD19+

/

/

NK Cells

Live single

Not eosinophils

mCD3-

mCD335+

/

Th Cells

Live single

Not eosinophils

mCD3+

mCD4+

mCD8-

Tc Cells

Live single

Not eosinophils

mCD3+

mCD4-

mCD8+

图片6.png



Experimental Case Study 3: Analysis of infiltrating immune cells in brain tissue (10-color panel)


Population

Gating step

Total Leukocytes

Live single

mCD45+

/

/

/

Neutrophils

Live single

mCD45+

mCD11b+

Ly6G+

/

Monocytes

Live single

Not Neutrophils

mCD11b+

Ly6C hi

/

Eosinophils

Live single

Not monocytes

mCD11b+

SSC-H hi

/

Macrophage

Live single

Not eosinophils

mCD11b+

F4/80+

/

T cells

Live single

Not eosinophils

mCD3+

mCD19-

/

B cells

Live single

Not eosinophils

mCD19+

mCD3-

/

Th Cells

Live single

Not eosinophils

mCD3+

mCD4+

mCD8-

Tc Cells

Live single

Not eosinophils

mCD3+

mCD4-

mCD8+

图片7.png


Advantages


  • Extensive experience processing different tissues.



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