Tumor-bearing Mouse Models

GPT possesses a vast collection of immune-deficient strains and target humanized strains on  both C57BL/6 and BALB/c backgrounds, coupled with an extensive resource of tumor cell lines. We offer tumor efficacy services using a variety of wild-type and humanized cells for both subcutaneous and orthotopic tumor studies. Furthermore, we supply a significant number of mouse models with commonly used human cell lines for subcutaneous tumor xenograft studies. By leveraging our comprehensive tumor induction data and robust experimental systems, we ensure that our customers receive stable and reliable oncology mouse models.


Service Contents

6 Types of Tumor Bearing Mouse Models


Serial Number

Strain Number

Strain Name

Strain 1

T064538

BALB/c-Nude+CDX

Strain 2

T064540

NCG+CDX

Strain 3

T064541

BALB/c(background)+Syngeneic Cell Lines

Strain 4

T064543

C57BL/6(background)+Syngeneic Cell Lines

Strain 5

T064544

BALB/c(background)+Immune Checkpoint Single Target + Syngeneic Cell Lines

Strain 6

T064545

C57BL/6(background)+Immune Checkpoint Single Target + Syngeneic Cell Lines


Cancer Cell Lines 


Cell origin

Type

Cell Lines

Syngeneic Cell Lines

Lymphoma

A20, J558

Melanoma

B16F0, B16F1, B16F10

Lung

LLC1, KLN 205

Liver

H22

Gastric Carcinoma

RENCA

Pancreas

PAN02

Colorectal

CT26.WT, MC38

Breast

4T1, EMT6

CDX

Leukemia

HL-60, Jurkat, K-562, MOLT-4, MV-4-11, NALM-6, SUP-B15, THP-1, OCI-AML-3

Lymphoma

U-937, JeKo-1, Daudi, Ramos, Raji, SU-DHL-10, U-2932

Multiple Myeloma

MM.1S, NCI-H929, MM.1R, RPMI 8226

Melanoma

A-375, COLO 829, SK-MEL-28, SK-MEL-5

Cutaneous Squamous Cell 

A-431

Sarcoma

HT-1080, SJSA-1

Glioma

U-87 MG, U-251 MG

Neuroblastoma

SK-N-BE(2)

Submandibular Gland Squamous Cell

A-253

Squamous Cell

CAL 27

Squamous Cell

FaDu

Esophageal Cancer

OE33

Esophageal Squamous Cell 

KYSE-150

Lung

A549, DMS 53, HCC827, NCI-H1703, NCI-H1975, NCI-H2122, NCI-H226, NCI-H292, NCI-H358, NCI-H446, NCI-H460, NCI-H82, NCI-H441, NCI-H69, NCI-H1650, SHP-77, NCI-H1299

Liver

Huh-7, Hep G2

Kidney

ACHN, 786-O

Pancreatic

AsPC-1, BxPC-3, Capan-1, CFPAC-1, MIA PaCa-2, PANC-1, SW 1990

Stomach

Hs746T, MKN45, NCI-N87, SNU-16, NUGC-4

Duodenal

HuTu 80

Colon

LS1034, SW948, SW480, SW620, COLO 320

Colorectal

COLO 205, HCT116, HT-29, HT-55, LoVo, LS174T, RKO, SW48

Bladder

RT112/84

Breast

BT-474, HCC1937, HCC1954, HCC1569, JIMT-1, MCF7, MDA-MB-231, MDA-MB-436, MDA-MB-453, MDA-MB-468, HCC70, T-47D

Ovarian

TOV-21G, SK-OV-3, OVCAR-3

Ovarian Teratoma

PA-1

Ovarian Clear Cell

ES-2

Endometrial

HEC-1-A

Prostate

LNCaP C4-2B, LNCaP Clone FGC, 22Rv1



Case


Cell Line 1

Inoculation Concentration

Background

Site

Grouping Time

Grouping 

Volume (mm³)

MDA-MB-231

106/100 μL/Piece

(1:1Matrigel)

NCG

3#

D10-D12

80-100


At GPT, the subcutaneous tumor implantation model is straightforward to operate, with a high success rate and easily accessible tumor volume measurements. Initially, tumors exhibit slow growth, which later transitions to exponential growth. 


图片17.png

Figure 1. NCG+ MDA-MB-231


Cell Line 2

Inoculation Concentration

Background

Site

Grouping Time

Grouping Volume (mm³)

LS174T

106/100 μL/site

(1:1 Matrigel)

Nude

3#

D5-D7

80-120


The LS174T model shows rapid tumor growth in its early stages and has a short overall experimental cycle, making it ideal for high-throughput drug screening. However, in the later stages, the tumors exhibit exponential growth and are susceptible to ulceration and scabbing. Handling the mice frequently might result in severe tumor ulceration and an increased risk of tumor nibbling, necessitating housing in individual cages.


图片18.png

Figure 2. BALB/c-Nude+LS174T 


Cell Line 3

Inoculation Concentration

Background

Site

Grouping Time

Grouping 

Volume (mm³)

A-375

5×106/100 μL/piece

(1:1 Matrigel)

NCG

3#

D6-D8

80-120


A-375 human melanoma cells were inoculated into NCG mice via subcutaneous injection, and tumor volumes were measured at different time points. The cell inoculation volume was 5×10cells per mouse, with or without the addition of matrix gel for comparison. The data are presented as Mean±SEM. The results demonstrate that an effective tumor model of human malignant melanoma can be established in NCG mice, with more uniform tumor growth observed when matrix gel is added. Similar to the previous models, the A-375 tumor exhibits slow initial growth, followed by exponential growth, with slight scabbing observed on the tumor surface and a low ulceration rate.

图片11.png图片12.png


Figure 3. NCG + A-375 


Cell Line 4

Inoculation Concentration

Background

Site

Grouping Time

Grouping Volume (mm³)

MC38

1×106/100 μL/piece

C57BL/6

3#

D7-D9

90-100


The MC38 model exhibits rapid growth in the later stages. Similar to the previous models, frequent handling of the mice may lead to severe tumor ulceration, requiring single-cage housing to minimize experimental errors.


图片20.png

Figure 4. C57BL/6 + MC38 


Cell Line 5

Inoculation Concentration

Background Mouse

Site

Grouping Time

Grouping Volume(mm³)

CT26.WT

1×106/100 μL/piece

BABL/c

1#

D9-D11

70-90


The CT26.WT model exhibits rapid early-stage growth and a short overall experimental cycle, making it suitable for high-throughput drug screening. However, in the later stages, the tumors are prone to ulceration and scabbing. Frequent handling of the mice may lead to severe tumor ulceration and a risk of tumor nibbling, thus requiring single-cage housing.


图片21.png

Figure 5. CT26.WT+BALB/c



GPT Advantages


  • 300+ cell lines are accompanied by tumor growth validation data..

  • Reliable, swift, and consistent mouse transportation system ensures accurate tumor volume delivery.

  • Stable experimental systems for both cell culture recovery and tumor implantation.

  • Rich resources of tumor cell lines.

  • Extensive experience in project experimentationadhering to regulatory requirements for new drug applications.

  • Clear risk alerts and compensation guidelines, effectively safeguard customer rights and interests.


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