Flow Cytometry Platform
The GPT Flow Cytometry Platform is equipped with multiple automatic tissue processing instruments and advanced flow cytometers, complemented by a team of highly skilled experts specializing in multi-color flow cytometry analysis. Over the years, the platform has successfully executed numerous flow cytometry projects, including immune cell analysis in different tissue types across various disease models, detection of tumor-infiltrating lymphocyte (TILs), and in vitro evaluation of antibody drugs.
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Services and Advantages
With a team of highly skilled experts specializing in multi-color flow cytometry analysis, the GPT Flow Cytometry Platform offers advanced flow cytometers and multiple automatic tissue processing instruments. This platform has a successful track record in executing various flow cytometry projects over the years. These projects include immune cell analysis in diverse tissue types across different disease models, detection of tumor-infiltrating lymphocytes (TILs), and the in vitro evaluation of antibody drugs.
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Immune Cell Profiling
Mouse immune cells can be affected by gene knockout, target modification, and drug treatment. The mouse immune system can be assessed by analyzing the proportions and quantities of T cells, B cells, NK cells, monocytes, granulocytes, macrophages, DC cells, and other cell populations in the peripheral blood and spleen. Through flow cytometry analysis of various immune cell types, we can identify the cellular populations affected by gene modifications or drug treament, thereby providing insights into their potential functions.
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Tumor Infiltrating Lymphocyte (TILs) Detection
Tumor Infiltrating Lymphocytes (TILs) are immune cells that exist in the tumor microenvironment. These cells primarily include cytotoxic T cells, helper T cells, regulatory T cells (Treg cells), NK cells, and macrophages.
The function of TILs encompass two primary aspects. Firstly, certain TILs cells are capable of recognizing and killing tumor cells, thereby exerting an anti-tumor effect. For example, Cytotoxic T-cells identify antigens on the surface of tumor cells and directly kill them by releasing cytotoxic molecules such as perforin and granzyme B. NK cells can also recognize and destroy tumor cells. Secondly, some immune cells within the tumor microenvironment, such as Treg cells, can suppress the immune response and promote the immune escape of tumors. Tumor-associated macrophages (TAMs) in the immune microenvironment have been found to be transformed into M2-type macrophages that promote tumor growth and metastasis, driving the development of tumors. -
Red Blood Cell and Platelet Detection
Engrafting HSC cells in NCG-X mice can reconstitute human red blood cells, and thus can be used for thalassemia studies by detecting red blood cells and hemoglobin in the bone marrow.
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Tissue Sample Analysis
Extensive experience processing different tissues，such as Spleen, Thymus, Lymph nodes, Kidney, Lung, Skin, Brain, Small intestine, Liver and Fat.
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Antibody Binding Assay
The antibody binding assay evaluates the binding between antibody-based drugs and target-positive cells, target-humanized mouse cells, or target-modified mouse cells. It determines the EC50 of the drug and provides a reference for in vivo efficacy by comparing it with positive control drugs.
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Cytometric Bead Array
The Cytometric Bead Array (CBA) is a multiplex protein quantification method based on the flow cytometry system, enabling the simultaneous detection of multiple markers within a single sample. It utilizes microspheres with different fluorescent intensities, each coated with specific antibodies (capture antibodies) that can recognize particular proteins. These microspheres interact with the sample (such as serum, plasma, culture supernatant, cell lysate, etc.) and with detection antibodies labeled with PE. The resulting complex is then analyzed by a flow cytometer. By comparing the PE fluorescence intensity to a standard curve generated through software analysis and reference standards, the CBA assay can perform quantification analysis of specific proteins. Compared to ELISA, the CBA assay is more sample-saving and convenient to operate.